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유산균의 생존율, 저장안정성, 내산성 또는 내담즙성을 증가시키는 방법
专利权人:
发明人:
CHOI, IN SUKKR,최인석,KIM, BYOUNG KOOKKR,김병국,MOON, SUNG HYUNKR,문성현,HEO, BO HYEKR,허보혜,SEO, MIN HOKR,서민호
申请号:
KR1020150082813
公开号:
KR1016055160000B1
申请日:
2015.06.11
申请国别(地区):
KR
年份:
2016
代理人:
摘要:
The present invention provides a method of increasing viability, storage stability, acid resistance, and bile resistance of the lactic acid bacteria after freeze-drying the lactic acid bacteria by adding proline to the lactic acid bacteria while lactic acid bacteria are being cultivated or after lactic acid bacteria are cultivated. A method of the present invention not only increases stabilities for external environmental stresses such as freeze-drying stability, storage stability and others of lactic acid bacteria themselves, but also remarkably improves acid resistance and bile resistance as stability indices of intestinal canal environment during intaking of lactic acid bacteria by containing a protection agent such as proline in lactic acid bacteria in a high concentration. Conventional technologies have dropped economic feasibilities and are limited in industrial applications by using complicated methods of constructing physical barriers on outer parts of the lactic acid bacteria by performing multistep coating processes such as a double coating process, a triple coating process and a quadruple coating process on protein, polysaccharide, porous polymer, edible oil and fat, etc. On the other hand, the present invention is an economical method which increases viability of the lactic acid bacteria during passing of the lactic acid bacteria through the gastrointestinal tract, is capable of exhibiting inherent functionality of the lactic acid bacteria in an intestine, and is capable of overcoming a problem of cost rise according to existing multistep coating processes by greatly increasing resistance to external physiochemical stresses of the lactic acid bacteria themselves.COPYRIGHT KIPO 2016본 발명은 유산균의 배양 중 또는 유산균을 배양한 후 프롤린을 첨가하여 유산균의 동결건조 후 생존율, 저장안정성, 내산성 및 내담즙성을 증가시키는 방법 및 상기 방법에 의해 제조된 유산균을 제공한다. 본 발명의 방법은 유산균체 내에 프롤린 등 보호제를 고농도로 함유하도록 함으로써 유산균 자체의 동결건조 안정성, 저장안정성 등 외부 환경 스트레스에 대한 안정성을 증가시킬 뿐만 아니라 유산균 섭취 시 장관환경 안정성의 지표인 내산성, 내담즙성을 현저히 향상시킨다. 종래 기술이 단백질, 다당
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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