SCHWALB CARSTEN,HUMMERICH DANIEL,BRUSER HEIKE,LIEBMANN BURGHARD,FEHR MARKUS
申请号：
NZ61584710
公开号：
NZ615847A
申请日:
2010.06.02
申请国别(地区):
NZ
年份:
2014
代理人:
摘要：
Disclosed is a precursor protein comprising a cleavable sequence of desired peptide (Pep) elements and auxiliary peptide (Aux&rsquo) elements of the general formula (Pep-Aux&rsquo)x or (Aux&rsquo-Pep)x where x >1, wherein: the Aux&rsquo elements are identical or different and comprise an amphiphilic alphahelix-forming peptide, said amphiphilic peptide comprising a sequence segment of at least seven peptidically linked amino acids capable of forming an amphiphilic alpha-helix, wherein the amino acid residues of said helix in its vertical projection are separated into a hydrophobic half and a hydrophilic half of the helix, the hydrophobic half of the helix having at least 3 adjacent, in the vertical projection, identical or different hydrophobic amino acid residues, and the hydrophilic half of the helix having at least 3 adjacent, in the vertical projection, identical or different hydrophilic amino acid residues the Pep elements are identical or different and comprise the amino acid sequence of identical or different peptide molecules having a sequence length of 5 to 70 amino acid residues the Pep elements are flanked by cleavage sequences which enable the Pep elements to be specifically cleaved out of the precursor protein and Pep is a cationic antimicrobial peptide and Aux&rsquo is an anionic peptide forming an amphiphilic alpha helix. Further disclosed is the use of an amphiphilic peptide as protective peptide for recombinantly producing an antimicrobial desired peptide different therefrom wherein said amphiphilic peptide comprises a sequence section of at least seven peptidically linked amino acids capable of forming an amphiphilic alpha-helix, wherein the amino acid residues of said helix in its vertical projection are separated into a hydrophobic half and a hydrophilic half of the helix, the hydrophobic half of the helix having at least 3 adjacent, in the vertical projection, identical or different hydrophobic amino acid residues, and the hydrophilic half of the
