A method for expanding human corneal stem endothelial cells includes: (a) providing an amniotic membrane with or without amniotic cells, wherein the amniotic membrane has an extracellular matrix (b) placing onto the amniotic membrane, a sheet of endothelial layer, or a cell suspension including human corneal endothelial stem cells and (c) culturing the corneal endothelial cells on the amniotic membrane for a duration sufficient for the corneal endothelial stem cells to expand to an appropriate area. The invention also relates to a method for creating a surgical graft for a recipient site of a patient using the method for expanding human corneal endothelial cells, and the surgical graft prepared therefrom.一種擴增人類角膜內皮幹細胞之方法,其包括:a)提供含或不含羊膜細胞之羊膜,其中該羊膜含細胞外間質;b)將一內皮層片或含人類角膜內皮幹細胞之細胞懸浮液置於該羊膜上;及c)於該羊膜上培養角膜內皮細胞一段足使該等角膜內皮幹細胞擴增至一適當面積之時間。本發明也係關於一種使用上述擴增人類角膜內皮細胞之方法產生施用於病患植入部位(recipient site)之外科移植物之方法,及由該方法製備之外科移植物。
