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METHOD FOR PRODUCING RETINAL TISSUE
专利权人:
SUMITOMO DAINIPPON PHARMA CO.; LTD.
发明人:
ANDO, SATOSHI,KURODA, TAKAO,SASAI, YOSHIKI (DECEASED)
申请号:
CA2998091
公开号:
CA2998091A1
申请日:
2016.09.08
申请国别(地区):
CA
年份:
2017
代理人:
摘要:
The present invention provides a method for producing retinal cells or retinal tissue, the method including the following steps 1-3: (1) a first step for culturing mammalian pluripotent stem cells in a medium that contains 1) pluripotency maintenance factor and 2) MEK inhibitor in the absence of feeder cells for a time not to exceed 30 days (2) a second step for suspension-culturing the cells obtained in the first step and forming aggregates of cells and (3) a third step for suspension-culturing the aggregates obtained in the second step in the presence of a BMP signaling pathway agonist, and obtaining aggregates that include retinal cells or retinal tissue.
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