A preparation method of adiposomes, and use thereof. Provided is a method for preparing adiposomes consisting of neutral lipids and a monolayer phospholipid membrane, comprising a1) vortexing phospholipid and neutral lipids in a buffer, centrifuging the resulting mixture, and collecting an upper liquid phase； a2) purifying the upper liquid phase twice or more by uniformly mixing the upper liquid phase with the buffer, layering the mixture, and collecting an upper liquid phase； and a3) uniformly mixing the upper liquid phase obtained in step a2) with the buffer, layering the mixture, and collecting a lower liquid phase in containing adiposomes. For the adiposomes prepared by the method, one or more resident proteins and/or functional proteins can be recruited to obtain artificial lipid droplets, and one or more apolipoproteins can be recruited to obtain artificial lipoproteins； and they all play important roles in preparing drugs and/or drug carriers.
