The present invention discloses a tumor necrosis factor-related apoptosis-inducing ligand variant, which is a fusion protein of a tumor necrosis factor-related apoptosis-inducing ligand and an F3 peptide. The F3 peptide is fused to the N-terminus or C-terminus of the tumor necrosis factor-related apoptosis-inducing ligand by a linker. The present invention also discloses a nucleotide sequence, as well as a recombinant vector and a recombinant bacterium comprising same, and also discloses a preparation method and use of the foregoing variant. The tumor necrosis factor-related apoptosis-inducing ligand variant is prepared by means of genetic engineering in the present invention, characterized in that its affinity for tumor cells, ability to induce apoptosis in tumor cells, tumor targeting property and in vivo anti-tumor effect are significantly better than those of the tumor necrosis factor-related apoptosis-inducing ligand. Accordingly, this variant displays good therapeutic effects on tumor treatment and has promising prospects in clinical application.
