TAKEDA PHARMACEUTICAL COMPANY LIMITED;KYOTO UNIVERSITY
发明人:
IKEYA, MAKOTO,池谷真,TOYOOKA, YAYOI,豊冈生,豊岡生
申请号：
TW107142689
公开号：
TW201940693A
申请日:
2018.11.29
申请国别(地区):
TW
年份:
2019
代理人:
摘要：
Provided as a technique for propagating neural crest cells without lowering the differentiation capacity is a method for producing neural crest cells that includes (1) a step for obtaining neural crest cells and (2) a step for suspension culturing the neural crest cells in medium containing a GSK3[beta] inhibitor and basic fibroblast growth factor (bFGF), the medium including a GSK3[beta] inhibitor of a concentration that presents an effect equivalent to the effect presented by CHIR99021 of a concentration from more than 1 [mu]M to less than 5 [mu]M.提供一種神經脊細胞之製造方法，作為用以在不降低分化能力下使神經脊細胞增殖的技術，該方法包含(1)得到神經脊細胞之步驟，與(2)於含有GSK3β抑制劑及鹼性纖維母細胞生長因子(bFGF)之培養基中將神經脊細胞懸浮培養之步驟，其中該培養基含有顯示與超過1μM且未達5μM之濃度的CHIR99021所顯示之效果同等效果的濃度之GSK3β抑制劑。
