William N. Drohan;Marian J. Drohan;Michael J. Griffith
发明人:
William N. Drohan,Marian J. Drohan,Michael J. Griffith
申请号：
US13459743
公开号：
US20120276079A1
申请日:
2012.04.30
申请国别(地区):
US
年份:
2012
代理人:
摘要：
Methods for producing cell lines with high levels of biologically active recombinant vitamin K dependent proteins are described. The transfected cell lines do not include heterologous genes for processing enzymes and are not subject to selection pressure such as methotrexate resistance. Cell lines producing Factor VII/VIIa and Factor IX are described. These cell lines can be used for isolation of Factor VII/VIIa and/or Factor IX for treatment of Hemophilia.
