您的位置: 首页 > 农业专利 > 详情页

USE OF BACILLUS THURINGIENSIS AND SPHAERICUS TO STIMULATE THE PRODUCTION OF PHENOLIC COMPOUNDS
专利权人:
B2B
发明人:
OLIVIER BESNARD
申请号:
FR0800719
公开号:
FR2929802B1
申请日:
2008.02.12
申请国别(地区):
FR
年份:
2012
代理人:
摘要:
Use of bacteria, proteins or peptides obtained from: Bacillus thuringiensis var kurstakiclones of Barjac and Lemille (serotype 3a and 3b), deposited at ATCC (American type culture collection) under number of 33679; Bacillus thuringiensis var israelensisclones of Barjac, deposited at ATCC under number of 35646; and Bacillus sphaericus Meyer and Neideclones, deposited at ATCC under numbers of 10208, is claimed. An independent claim is included for a process for obtaining a composition containing bacteria, proteins or peptides, comprising: culturing the bacteria using a culture medium, which is relatively simple (MS) of which composition comprises potassium dihydrogen phosphate (2.5 g/l), dipotassium dihydrogen phosphate (2.5 g/l), diammonium hydrogen phosphate (1 g/l), magnesium sulfate heptahydrate (0.2 g/l), manganese sulfate heptahydrate (7 mg/l), ferrous sulfate heptahydrate (0.01 g/l) and calcium chloride (10 mg/l), adding standard saline solution of 1% D-glucose and adjusting the pH to 7, sterilizing the medium by autoclaving at 120[deg] C for 20 minutes, inoculating a preculture of bacteria in MS medium for 15 hours at 30[deg] C under agitation, and placing a flask containing the bacteria in culture at 37[deg] C under gentle rotative agitation at 150 revolutions per minute (rpm) for 5-7 days; and (b) separating the bacteria and proteins at 4[deg] C, comprising centrifuging the contents of each flask at 4500 rpm for 30 minutes, washing the pellet in sodium chloride solution (1M) twice with distilled water containing phenylmethylsulfonyl fluoride (1 mM) and EDTA (10 mM) and then resuspended in a demineralized water and purifying the protein part of a discontinuous sucrose gradient by Thomas and Ellar method (1983) [ultracentrifugation at 25000 rpm at 48[deg] C for 16 hours], concentrating into protein, which is determined by the Bradford method after alkaline solubilization of the extract and electrophoresis denaturing conditions (sodium dodecyl sulfate-polyacryl
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

意 见 箱

匿名:登录

个人用户登录

找回密码

第三方账号登录

忘记密码

个人用户注册

必须为有效邮箱
6~16位数字与字母组合
6~16位数字与字母组合
请输入正确的手机号码

信息补充