THE UNITED STATES OF AMERICA; AS REPRESENTED BY THE SECRETARY; DEPARTMENT OF HEALTH AND HUMAN SERVICES
发明人:
GAO, WEI,HASSAN, RAFFIT,HO, MITCHELL,PASTAN, IRA H.,PHUNG, YEN T.,ZHANG, YIFAN
申请号：
CA2882753
公开号：
CA2882753A1
申请日:
2013.08.16
申请国别(地区):
CA
年份:
2014
代理人:
摘要：
Described herein is the use of rabbit hybridoma technology, along with a panel of truncated mesothelin domain fragments, to identify anti-mesothelin mAbs that bind specific regions of mesothelin. In one aspect of the present disclosure, the rabbit mAbs bind an epitope that is not part of Region I. In particular, the identified mAbs (YP187, YP223, YP218 and YP3) bind either Region II (391-486), Region III (487-581) or a native conformation of mesothelin with subnanomolar affinity. These antibodies do not compete for binding with the mesothelin- specific immunotoxin SS1P or mesothelin- specific antibody MORAb-009. In another aspect, disclosed is a high-affinity rabbit mAb that binds Region I of mesothelin (YP158). YP158 binds native mesothelin protein in cancer cells and tissues with high affinity and specificity.
