Applications of kelimycin in mycobacterium tuberculosis infection resistance. The applications comprise the following main steps: by using clinical frequently-used antitubercular drugs isoniazide and rifamycin as contrast, detecting the activity of kelimycin in mycobacterium tuberculosis resistance by using an absolute concentration method. The result indicates that the activity of kelimycin on clinically separated mycobacterium tuberculosis comprising drug-resistance bacteria is remarkably superior to those of the clinical frequently-used contrast drugs isoniazide and rifamycin, and applications of kelimycin in the preparation of drugs for treating tubercle bacillus infected diseases are expected to be developed.