The invention relates to materials and methods of conjugating a water soluble fatty acid derivative to a therapeutic protein comprising contacting the therapeutic protein with an activated water soluble fatty acid derivative under conditions that allow conjugation.本發明係關於偶聯水溶性脂肪酸衍生物與治療蛋白質之材料及方法,其包括使治療蛋白質與活化之水溶性脂肪酸衍生物在允許偶聯之條件下接觸。