Myung Hyun JANG,Min Young KIM,Dae Jin KIM,Sung Youb JUNG,Se Chang KWON
申请号：
US14772493
公开号：
US20160008484A1
申请日:
2014.03.05
申请国别(地区):
US
年份:
2016
代理人:
摘要：
Provided is a method for preparing a conjugate by linking a physiologically active polypeptide, a non-peptidyl polymer linker, and an immunoglobulin constant region via a covalent bond. The method for efficiently preparing the physiologically active polypeptide conjugate uses a salt in a coupling reaction to solve the problem of low production yield during preparation of the physiologically active polypeptide conjugate. The conjugate of physiologically active polypeptide-non-peptidyl polymer-immunoglobulin constant region can be produced with high purity and yield by the subject method. Due to the method for preparing the physiologically active polypeptide conjugate, production costs can be reduced. Therefore, the method can be used to develop long-acting formulations of physiologically active polypeptides, which have improved industrial applicability and drug regulation compliance.
