Recombinant plasmid DNA pMSIN4, encoding a hybrid polypeptide comprising human insulin precursor, E. coli strain BL21 (DE3) / pMSIN4 - producer of recombinant human insulin, the method for the recombinant human insulin production
The invention refers to biotechnology, particularly genetic engineering, and can be used in the production of recombinant human insulin.The technical result, at which the invention is aimed, is to increase the quality and quantity of yield of the desired product - recombinant human insulin, as well as to simplify the industrial production of recombinant human insulin.The result is achieved by constructing a recombinant plasmid pMSIN4, encoding a hybrid polypeptide - a precursor of human insulin, a strain VL21 (DE3) / pMSIN4 - producer of recombinant human insulin, as well as by optimizing the way of the industrial production of recombinant human insulin.The inventions therefore allows to simplify the process of the industrial production of the high purity recombinant human insulin, as well as to increase the yield of the desired product.
