САСТРИ-ДЕНТ Лакшми (US),ЭЙНЛИ У. Майкл (US),СЭМЬЮЭЛ Джаякумар П. (US),ЦАО Цзэхуэй (US),ШЭНЬ Лю И. (US),ДЬЮЭС Кристи М. (US)
申请号：
RU2016112305
公开号：
RU2016112305A
申请日:
2014.09.03
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
The present disclosure provides methods for detecting and identifying plant events that contain donor sequences inserted precisely into targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce a first amplicon from donor sequences inserted in the reverse orientation, amplifying the first amplicon with a second round of PCR and detecting the presence of the second amplicon, wherein the production of the first and second amplicon indicates the presence of the site specific integration event.
