The present application relates to a machine-readable medium having machine-executable instructions configured to perform a method for analyzing the ophthalmic anatomy of a patient posterior to the cornea. The method comprises scanning (1110) a focus of a femtosecond laser beam along a path within the patient's eye, wherein at least a portion of the path is disposed posterior to the patient's cornea, and wherein the path includes a first focus location and a second focus location, acquiring (1120) a first reflectance image associated with the focus disposed at the first focus location, acquiring (1130) a second reflectance image associated with the focus disposed at the second focus location, and determining (1140) the presence or absence of an ophthalmic anatomical feature of the eye based on a comparison between the first reflectance image and the second reflectance image.
