Disclosed is a photo-stimulation method and device with a light mixture. The method includes the following steps: providing a light-emitting diode (LED) illuminant which is a combination of a yellow LED and a red LED and illuminating a subject by the LED illuminant to promote collagen synthesis, to suppress microbial growth, or to inhibit melanin synthesis, wherein the yellow LED is in an illuminance range from 1,000 to 3,500 lux, the red LED is in an illuminance range from 6,000 to 9,500 lux, and the number ratio of the yellow LED to the red LED is 0.5-2:0.5-2.
