E. I. DU PONT DE NEMOURS AND COMPANY;Pioneer Hi-Bred International
发明人:
Karen E. Broglie,Mani Muthalagi,Kevin Kriss,Albert L. Lu,James K. Presnail
申请号：
US13791596
公开号：
US09686995B2
申请日:
2013.03.08
申请国别(地区):
US
年份:
2017
代理人:
摘要：
Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
