The present invention provides a biosensor with finger-positioning function, comprising: a bottom plate, a cover, and a test-strip socket wherein the cover mounted on the bottom plate has a front end provided with a guiding plate for positioning a finger, and the guiding plate has a center line and a guiding surface, in which the center line passes through a center of the guiding surface and the test-strip socket is formed at the front end of the cover. When using the biosensor, the user can rest his/her wrist and palm on the cover of the biosensor and rest the punctured finger on the guiding surface provided at the front end of the cover to align the test strip with the blood drop on the finger so as to simplify the measurement.
