Disclosed is a method for producing a three-dimensional polymer-metal complex microstructure including forming a polymer structure by stereolithography using a photocurable resin having a reactive group X and dipping it in a liquid of a metal-containing nanoparticle having a reactive group X' which is bound to the reactive group X, thereby forming a metal-containing layer on the polymer structure through binding the reactive group X and the reactive group X'. According to this method, it is possible to produce a polymer-metal complex structure having a steric structure and to produce a three-dimensional polymer-metal complex microstructure which does not denature biomolecules in a metal complexation process.