A self-contained biological indicator (“SCBI”) is disclosed. The SCBI may include a first ampule and a second ampule. The first ampule may contain a first volume of a first growth medium, and the second ampule may contain a second volume of a second growth medium. The SCBI is configured such that the first ampule may be broken before the second ampule. The SCBI may be analyzed for changes in fluorescence of the first growth medium. Then, the SCBI may be analyzed for changes in a color of the second growth medium.所揭示者係一種自供式生物指示器(「SCBI」)。該SCBI可包括一第一安瓿及一第二安瓿。該第一安瓿可含有一第一體積的一第一生長介質，且該第二安瓿可含有一第二體積的一第二生長介質。該SCBI經組態使得該第一安瓿可在該第二安瓿之前被打破。可針對該第一生長介質的螢光變化而分析該SCBI。然後，可針對該第二生長介質的顏色變化而分析該SCBI。
