#$%^&*AU2020100624A420200604.pdf#####ABSTRACT The invention discloses a method for screening cold-resistant cotton varieties and application thereof. The method comprises the following steps of (1) seeding cotton seeds of different varieties; after the seeding for two days, realizing young seedling emergence; removing young seedlings with nonuniform growth conditions in the fourth day; maintaining the continuous growth of other young seedlings; (2) realizing flat spreading of cotyledons after the young seedlings grow to the seventh day; performing cold adaptation on the young seedlings; then, performing cold treatment; next, performing hot adaptation and restoration treatment; counting the survival rate and counting the cotyledon cold damage level; next, calculating the form index; testing the relative electrical conductivity of detached leaves when the cold treatment is performed for 72 hours; and evaluating the cold resistance according to the form index or the relative electrical conductivity. 1DRAWINGS FIG. 1 FIG. 2 1