Commonwealth Scientific and Industrial Research Organisati
发明人:
Waterhouse, Peter,Wang, Ming Bo,Graham, Michael
申请号：
ES10183703
公开号：
ES2624549T3
申请日:
1999.04.07
申请国别(地区):
ES
年份:
2017
代理人:
摘要：
A method for reducing the phenotypic expression of a nucleic acid of interest, which can be expressed in a plant cell, comprising the step of introducing into said cell a chimeric DNA, which is stably integrated into the nuclear genome of said plant cell, comprising said chimeric DNA the following operatively linked parts: a) a promoter, operative in said plant cell； b) a region of DNA that, when transcribed, gives an RNA molecule with a nucleotide sequence comprising i. a sense nucleotide sequence of less consecutive nucleotides having 100% sequence identity with at least part of a coding region of said nucleic acid of interest； and ii. an antisense nucleotide sequence that includes at least 20 consecutive nucleotides, which has 100% sequence identity with the complement of said at least 20 consecutive nucleotides of said sense nucleotide sequence； wherein the RNA is capable of forming an artificial hairpin RNA structure with a double-stranded RNA stem by base pairing between regions with sense nucleotide and antisense sequence so that at least said 20 consecutive nucleotides of the sequence of sense are paired by bases with said 20 consecutive nucleotides of the antisense sequence； wherein said chimeric DNA comprises one or more introns in said region of DNA that, when transcribed, gives an RNA molecule.Un método para reducir la expresión fenotípica de un ácido nucleico de interés, que se puede expresar en una célula vegetal, que comprende la etapa de introducir en dicha célula un ADN quimérico, que se integra establemente en el genoma nuclear de dicha célula vegetal, comprendiendo dicho ADN quimérico las siguientes partes enlazadas operativamente: a) un promotor, operativo en dicha célula vegetal； b) una región de ADN que, cuando se transcribe, da una molécula de ARN con una secuencia nucleotídica que comprende i. una secuencia nucleotídica de sentido de menos nucleótidos consecutivos que tiene 100% de identidad de secuencia con al menos parte de una región codif
