A decontamination assembly, for pharmaceutical applications includes at least one chamber to be decontaminated (4) having a decontamination liquid storage tank (36) by which several atomizers (A, B, C. D) disposed in the chamber to be decontaminated (4) for producing a decontaminant aerosol can be supplied with decontamination liquid using valves (27) which can be controlled by electronic control, the atomizers (A, B, C, D) being in fluid communication with a ring conduit (32) having an inlet (33) and a separate outlet (34) by the valves (27), decontamination liquid from the decontamination liquid storage tank (36) being conveyable into the inlet of the ring conduit by a pump (35) and decontamination liquid flowing out of the outlet of the ring conduit back into the decontamination liquid storage tank (36), and a pressure adjustment mechanism (37) for keeping the pressure in the ring conduit (32) at a predefined liquid pressure range assigned to the ring conduit (32). A measuring mechanism (40) for determining a total volume of decontamination liquid dispensed to the atomizers (A, B, C, D) during a decontamination cycle includes a weighing mechanism (41) for determining a decontamination liquid volume in the decontamination liquid storage tank (36).