Bradley L. Pentelute,Xiaoli Liao,Amy E. Rabideau,Jingjing Ling,Rocco L. Policarpo
申请号：
US14402915
公开号：
US09731029B2
申请日:
2013.05.21
申请国别(地区):
US
年份:
2017
代理人:
摘要：
Proteins containing a C-terminal thioester are important intermediates in semi-synthesis. Currently there is one main method for the synthesis of protein thioesters that relies upon the use of engineered inteins. The invention involves, in some aspects a method, utilizing Sortase A, for preparation of recombinant proteins containing a C-terminal αthioester. This new method for double ligatation is useful for synthesizing new or naturally occurring molecules such as a protein thioester.
