A method of cultivating Antrodia cinnamomea comprises: providing Antrodia cinnamomea； providing a pumpkin； and inoculating Antrodia cinnamomea into the pumpkin, followed by culturing for 3-6 weeks at 24-26℃ so that Antrodia cinnamomea can grow up according to nutrients of the pumpkin. Accordingly, the time for cultivating Antrodia cinnamomea is decreased and the cost for cultivating Antrodia cinnamomea is also diminished due to the method being free from the expensive apparatus or the wood segments difficult to obtain. Moreover, cultivating Antrodia cinnamomea by the nutrients provided by the pumpkin can obtain Antrodia cinnamomea with triterpenoids, and therefore the Antrodia cinnamomea rich in nutrients can be rapidly and produced.一種牛樟芝培養方法，包含：提供一牛樟芝；提供一南瓜；及將該牛樟芝植入該南瓜，於24~26℃之溫度下，培養3~6週，使該牛樟芝利用該南瓜所含有之營養成分生長。藉由以該南瓜作為該基質以供該牛樟芝生長，可以縮短該牛樟芝的培養時間，並且免除使用昂貴的設備或難以取得的椴木來培養，藉此降低成本，又，藉由該南瓜之營養成分來供該牛樟芝生長，可以獲得具有三萜類的該牛樟芝，以達到快速並大量生產該牛樟芝及提升該牛樟芝營養價值的功效。
