A 3D organoid is used for diagnosis or assay, wherein the 3D organoid is constructed from a tumor cell. The tumor cell in the 3D organoid is from a cell line, from circulating tumor cells isolated from a patient, or from a tumor tissue. The 3D organoid is constructed using an aqueous gel material and an adhesion molecule. The aqueous gel material is hydrogel. The adhesion molecule is ICAM-1 or galectin-3. The 3D organoid is constructed by forming a scaffold with an aqueous gel material, followed by adding a mixture of the tumor cell and an adhesion molecule.本發明係揭示用於診斷或分析之3D類器官，其中該3D類器官係構築自腫瘤細胞。該3D類器官中之腫瘤細胞係來自細胞株、來自分離自患者之循環腫瘤細胞，或來自腫瘤組織。該3D類器官係使用水性凝膠材料及黏附分子構築。該水性凝膠材料係水凝膠。該黏附分子係ICAM-1或半乳糖凝集素-3。該3D類器官藉由形成具有水性凝膠材料之支架，接著添加該腫瘤細胞及黏附分子之混合物來構築。
