STENMARK, PAL ERIK GUSTAV,BERNTSSON, RONNIE PER-ARNE,DONG, MIN,PENG, LISHENG
申请号：
CA2874587
公开号：
CA2874587A1
申请日:
2013.03.13
申请国别(地区):
CA
年份:
2013
代理人:
摘要：
Disclosed herein are botulinum neurotoxin (BoNT) polypeptides with a modified receptor binding domain of Clostridial botulinum serotype B (B-Hc), comprising one or more substitution mutations corresponding to substitution mutations in serotype B, strain 1, V1118M； Y1183M； E1191M； E1191I； E1191Q； E1191T； S1199Y； S1199F； S1199L； SI 20 IV； or combinations thereof. Specific combination mutations include El 191M and S1199L, E1191M and S1199Y, E1191M and S1199F, E1191Q and S1199L, E1191Q and S 1199Y, or E 1191 Q and S 1199F. Other substitution mutations are also disclosed. Isolated modified receptor binding domains, chimeric molecules, pharmaceutical compositions, and methods of using the same are also disclosed.
