The present disclosure generally relates to a microfluidic device and a method for cell separation of a blood sample. The microfluidic device comprises a set of inlets for receiving the blood sample； a set of outlets fluidically connected to the inlets, comprising a first waste outlet, an output sample outlet, and a second waste outlet； and a set of microfluidic channels fluidically connecting the inlets to the outlets. The set of microfluidic channels comprises a spiral microfluidic channel for receiving the blood sample from the inlets； and an arcuate microfluidic channel fluidically connected to the spiral microfluidic channel. The spiral microfluidic channel bifurcates into the arcuate microfluidic channel and first waste outlet from an inner side and outer side of the spiral microfluidic channel respectively. The arcuate microfluidic channel bifurcates into the output sample outlet and second waste outlet from an inner side and outer side of the arcuate microfluidic channel respectively. The output sample outlet is arranged for collecting an output sample that is substantially depleted of red blood cells relative to the blood sample.本揭示大致上有關一種用於血液樣本之細胞分離的微流體裝置及方法。該微流體裝置包含一組用於接收該血液樣本之入口；一組流體連接至該入口之出口，其包含一第一廢物出口、一輸出樣本出口及一第二廢物出口；及一組流體連接該入口至該出口之微流體通道。該組微流體通道包含用於接收從該入口過來之血液樣本之一螺旋微流體通道；及流體連接至該螺旋微流體通道之一弧形微流體通道。該螺旋微流體通道分別從該螺旋微流體通道之內側及外側分岐成該弧形微流體通道及第一廢物出口。該弧形微流體通道分別從該弧形微流體通道之內側及外側分岐成該輸出樣本出口及第二廢物出口。該輸出樣本出口配置成用於收集相對於該血液樣本實質上耗減紅血球的輸出樣本。
