Apparatuses and methods for performing and aiding venipuncture. The apparatuses of the present invention provide the medical practitioner with an improved tool for performing venipuncture methods. The apparatus preferably includes a handle that is connected to a plurality of tines that run roughly parallel to one another. During use, the tines of the apparatus are pressed against the skin of the patient such that they sit astride the vein to be accessed. The distance between the various tines of the present apparatuses varies so as to be able to accommodate a variety of vein widths. The apparatuses of the present invention may have tines at either one or both ends of the body of the apparatus. The apparatuses of the present invention may be used with a variety of vein accentuating devices, such as those that employ light to accentuate the vein to be punctured.