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СПОСОБ ПОЛУЧЕНИЯ ЭЛИТНОГО ПОСАДОЧНОГО МАТЕРИАЛА КАРТОФЕЛЯ
专利权人:
Лукша Сергей Анатольевич (RU)
发明人:
Лукша Сергей Анатольевич (RU)
申请号:
RU2014130030
公开号:
RU2014130030A
申请日:
2014.07.22
申请国别(地区):
RU
年份:
2016
代理人:
摘要:
A method of producing elite planting material potatoes, comprising culturing improved planting in vitro potato material by mikrocherenkovaniya on a nutrient medium, obtaining regenerated plants and their subsequent planting in the ground, characterized in that the pre-selected free of virus parent potato tubers with the number of holes of at least 6 -7 pieces produce buds sprouting stimulation by cross cut tuber apical portion and a longitudinal incision of the tuber with stolonovoy far necks notched tubers delayed in the dark at a temperature of 12-16 degrees and relative humidity of 75-85% of the ozone-air mixture until the sprouts 8-15 mm, whereupon tubers finally separated, and placed in soil, sprinkled nutrient substrate, and after the appearance sprouting by their shade cylindrical with open ends of the pipes, under illumination 14-17 hours a day, temperature of 20-25 degrees and humidity ozone-air mixture of 75-85% until the intense color of potato shoots characteristic of a particular class, then roizvodyat cuttings to the apical, middle and basal part of the inoculated on a medium for prescribing Murasa-Skoog medium, with the planted cuttings treated periodically moving the radiation flux in the red spectral region, wherein the growing of cuttings is carried out at temperature 20-25 degrees during the day, if svetodne to 15 hours and a temperature of 15-20 degrees at night, and after receiving the formed cuttings plants produce only the apical part of the plant, followed by cultivation in a nutrient medium, and the remaining test tube pa The walls are planted in climaticСпособ получения элитного посадочного материала картофеля, включающего культивирование оздоровленного посадочного материала картофеля in vitro путем микрочеренкования на питательную среду, получение растений-регенерантов и их последующую высадку в грунт, отличающийся тем, что предварительно отбирают свободные от вирусов материнские клубни картофеля с количеством глазков не менее 6-7 штук, пр
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