A 11-dehydrosinulariolide isolating method from culturedSinularia flexibilisincludes the steps of: utilizing ethanol (alcohol) to extract an extracted solution fromSinularia flexibilis utilizing methylene chloride/water to partition a methylene chloride layer and a water layer separating the methylene chloride layer by column chromatography, and gradient eluting the methylene chloride layer by a first elution buffer so as to obtain a number of fraction layers separating a designated layer of the fraction layers, and eluting the designated layer by a second elution buffer so as to obtain the 11-dehydrosinulariolide compound. The 11-dehydrosinulariolide compound is for use in treatment of Neurodegenerative diseases.一種自養殖型柔軟指形軟珊瑚分離天然物11-dehydrosinulariolide方法包含步驟如下:利用乙醇自柔軟指形軟珊瑚進行萃取,以提取一萃取液;利用二氯甲烷/水將該萃取液進行分配,以取得一二氯甲烷層及一水層;將該二氯甲烷層以管柱層析法進行初步分離,並利用一第一沖提溶液進行沖提,以取得數個劃分層提取液;將適當選擇該劃分層提取液之一指定分層進行分離,並利用一第二沖提溶液進行沖提,以取得11-dehydrosinulariolide。該天然物11-dehydrosinulariolide用於治療神經退化性疾病。S1...第一執行步驟S2...第二執行步驟S3...第三執行步驟S4...第四執行步驟
