federalnoe gosudarstvennoe byudzhetnoe uchrezhdenie "Natsionalnyj meditsinskij issledovatelskij tsentr akusherstva, ginekologii i perinatologii imeni akademika V.I. Kulakova" Ministerstva zdravookhraneniya Rossijskoj Federatsii
发明人:
Uvarova Elena Vitalevna,Donnikov Andrej Evgenevich,Kazakova Anna Vladimirovna,Komarova Marina Valerievna,Limareva Larisa Vladimirovna,Bezrukova Alina Andreevna
申请号：
RU20160138679
公开号：
RU2671561(C2)
申请日:
2016.09.30
申请国别(地区):
俄罗斯
年份:
2018
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention relates to medicine, namely to gynecology. Method for centile evaluation of the microcenosis of the vaginal mucosa in girls is proposed by selecting a biomaterial from the mucosa of the lateral wall of the vagina behind the physiological hole of the hymen by scraping with a disposable urogenital probe. Real-time PCR method is used to determine the number of genome equivalents and the proportion of total bacterial mass of Lactobacillus spp., obligate anaerobes and facultative anaerobes and compared with the centile interval of the corresponding group of microorganisms obtained for the corresponding stage of sexual maturation of the girl on the Tanner scale. For 1 and 2 stages of sexual maturation according to Tanner, the prevalence of the proportion of the total amount of obligate anaerobes over the Lactobacillus spp share is considered to be the norm. For the 3stage of sexual maturation, the normocoenosis of the vagina changes toward the predominance of Lactobacillus spp.
