A sterilization system for a sterile liquid transfer port system includes an isolation wall having a first and second side, an alpha assembly spanning a port in the isolation wall between the first and second sides of the isolation wall and a beta assembly configured for connection with the alpha assembly from the first side of the isolation wall for sterile transfer of a product. The system further includes a docking cover on the first side of the isolation wall which can mate to the beta assembly during a sterilization process.