A blood plasma and blood cell separation device includes a first body, a blood plasma separation membrane and a second body. The first body has an injection port. The blood plasma separation membrane is disposed on the first body. The second body is movably assembled to the first body and has a collecting stage. The collecting stage has a collecting surface and a sampling slot on the collecting stage. When a blood enters the blood plasma separation membrane from the injection port of the first body, a blood plasma in the blood plasma separation membrane is separated by a capillary force between the collection stage of the second body and the blood plasma separation membrane, and the blood plasma enters in the sampling slot by a relative movement between the first body and the second body.一種血漿血球分離裝置,包括一第一本體、一血漿分離薄膜以及一第二本體。第一本體具有一注入口。血漿分離薄膜配置於第一本體內。第二本體可移動地組裝於第一本體上,且具有一收集載台。收集載台上具有一收集面以及一取樣槽。當一血液從第一本體的注入口進入血漿分離薄膜時,第二本體的收集載台與血漿分離薄膜之間藉由毛細力而使血漿分離薄膜中的一血漿脫離,並藉由第一本體與第二本體之間的一相對運動,而使血漿進入取樣槽內。100:血漿血球分離裝置 110:第一本體 112:注入口 120:血漿分離薄膜 130:第二本體 131:第二面 132:收集載台 133:收集面 134:取樣槽 135:第二連接部/凸塊