Thomas M. Malvar;Larry A. Gilbertson;Elysia K. Krieger;Nancy M. Houmard;Shihshieh Huang;Edwards M. Allen
发明人:
Edwards M. Allen,Larry A. Gilbertson,Nancy M. Houmard,Shihshieh Huang,Elysia K. Krieger,Thomas M. Malvar
申请号：
US12832942
公开号：
US09000264B2
申请日:
2010.07.08
申请国别(地区):
US
年份:
2015
代理人:
摘要：
The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
