ХУАН Сян (US),МАКНЕЙЛЛ Томас (US),ШВАЙНЕР Майкл (US)
申请号：
RU2013141957/10
公开号：
RU2013141957A
申请日:
2012.02.14
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
1. A method for producing a library of small interfering RNA (siRNA), which involves the synthesis of a plurality of RNA molecules, wherein each RNA molecule comprises (a) a seed sequence containing random nucleotides and nezatravochnuyu sequence comprising certain nucleotides or (b) seed sequence comprising nucleotides specific to one or more miRNA seed sequence of the target organism or organisms related to the organism of target and nezatravochnuyu sequence comprising certain nukleotidy.2. The method of claim 1, wherein the synthesis involves the development in silico.3. A method according to claim 1 or 2, wherein each RNA molecule of the plurality of RNA molecules comprises at least about 17-19 nucleotides, and wherein the seed comprises a sequence 6-8 nukleotidov.4. A method according to claim 3, wherein each of the plurality of RNA molecules the RNA molecule comprises 21 nucleotides wherein the seed sequence comprises nucleotides occupying position 2-8 of the RNA molecule, and wherein nezatravochnaya sequence comprises nucleotides occupying positions 1 and 9-21 molecules RNK.5 . A method according to claims 1, 2 and 4, wherein nezatravochnaya sequence comprises (a) a consensus sequence of miRNA or (b) a consensus sequence of the miRNA plant pest or pathogen, or from the organism, pest or pathogen related rasteniya.6. A method according to claim 5, wherein the plant pest is a nematode or nasekomoe.7. The method of claim 6 wherein the nematode is soybean cyst nematode (Heterodera glycines), or the insect is Western corn beetle (Diabrotica virgifera) .8. A method according to claim 7, wherein nezatravochnaya sequence (a) is to
