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TAL-MEDIATED TRANSFER DNA INSERTION
专利权人:
J.R. SIMPLOT COMPANY
发明人:
ROMMENS, CAIUS M.,DUAN, HUI,WEEKS, TROY J.
申请号:
CA2891956
公开号:
CA2891956A1
申请日:
2013.11.19
申请国别(地区):
CA
年份:
2014
代理人:
摘要:
The invention relates to methods for stably integrating a desired polynucleotide into a plant genome, comprising transforming plant material with a first vector comprising nucleotide sequences encoding TAL proteins designed to recognize a target sequence transforming the plant material with a second vector comprising (i) a marker gene that is not operably linked to a promoter ("promoter-free marker cassette") and which comprises a sequence homologous to the target sequence and (ii) a desired polynucleotide and identifying transformed plant material in which the desired polynucleotide is stably integrated.
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