Provided is a simple and easy to operate device for collecting and extracting fecal samples. The collection device of the invention comprises a top cap mounted with a sample collection means, a middle cap inserted with an elastic sealing funnel, and a bottom container having only one opening. The device can collect fecal samples with a quantitative sampling accuracy comparable to that of the conventional weighing method. It can also he easily adapted to automated multi-sample extraction and immunoassays.