LAS VEGAS;THE BOARD OF REGENTS OF THE NEVADA SYSTEM OF HIGHER EDUCATION ON BEHALF OF THE UNIVERSITY OF NEVADA
发明人:
SCHILLER, MARTIN R.,BENJAMIN, RONALD
申请号：
CA3031712
公开号：
CA3031712A1
申请日:
2017.07.14
申请国别(地区):
CA
年份:
2018
代理人:
摘要：
Disclosed are compositions and methods for studying a Tat gene. Specifically, the disclosure provides a vector comprising a double- stranded nucleic acid construct which comprises a Tat gene and a green fluorescent protein (GFP) reporter element, and further wherein the double-stranded nucleic acid construct comprising AAVS1 (adeno-associated virus integration site, a safe harbor locus) arms that flank on both sides of the Tat gene and the reporter element for integration at the human AAVS1 site by homologus recombination. Further provided are methods of using a cell comprising the vector for studying the effects of exogenous conditions on expression of the Tat gene.
