A device combining laser technology with ozone and its use in a method to treat fungal infections is provided. The invention relates to a device to apply laser technology at different wavelengths in combination with ozone in a gaseous state. To do so it uses a plurality of laser emitters arranged in an electronic board, the light from which is radiated on a treatment chamber where a sealed bag containing the affected limb is introduced, and which is pumped with a pre-established concentration of ozone by means of an ozone generating system. The device is used as an effective method to treat fungal infections.