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Plant dbn9858 herbicide tolerant corn and Nucleic Acid Sequence and Method for Detecting
专利权人:
BEIJING DABEINONG TECHNOLOGY GROUP CO., LTD.;BEIJING DABEINONG BIOTECHNOLOGY CO., LTD.
发明人:
YUEJING KANG,MINGXIN GUO,HAILI LIU,CHENGWEI ZHANG,DERONG DING,GUOWEI JIAO,XUESONG WEI,BO TANG,ZULING XIA,GUANJUN XIONG,LIANG XU,XIAOMING BAO
申请号:
ARP160101205
公开号:
AR104436A1
申请日:
2016.04.28
申请国别(地区):
AR
年份:
2017
代理人:
摘要:
Claim 1: nucleic acid molecule, wherein the nucleic acid sequence includes at least 11 consecutive nucleic acid sequences or their auxiliary sequences in SEQ No. 3, and / or at least 11 consecutive nucleic acid sequences or their auxiliary sequences in SEQ No. 4: Exactly Requirement 5: a method for detecting the presence of DNA in dn9858 transgenic maize in one sample, including: connecting the sample with at least two originators to amplify a relevant amplification product in nucleic acid amplification reaction;Carry out nucleic acid amplification reaction and detect the presence of relevant amplification products; in these products, the relevant amplification products include at least 11 nucleotides in SEQ ID 3, or its auxiliary sequence, And / or at least 11 consecutive nucleotides in SEQ ID 4 or its auxiliary sequence. Claim 15: the DNA detection package comprises at least one DNA molecule, wherein the DNA molecule comprises at least 11 consecutive nucleosides in the sequence corresponding to SEQ ID 3 or its auxiliary sequence,And (or) at least 11 consecutive nucleotides in the corresponding sequence of SEQ ID 4 or its auxiliary sequence are useful as detectors for DNA originators or specific dn9858 gene gene gene gene events or their offspring. Requirement 19: a method for corn production that is resistant to the herbicide glyphosate and / or glutamic acid herbicide, including: encoding glyphosate resistant EPSPs protein and / or nucleic acid sequence into glucose resistant pat protein using a series of nucleic acids; and A series of nucleic acids are located in a specific region of the maize plant genome,The nucleic acid sequence of specific region shall be at least the nucleic acid sequence selected from the sequence listed in SEQ ID No.1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.6 and SEQ ID No.7; The method of inputting nucleic acid sequence in SEQ ID 5 into maize plant genome, requirement 23: a method of controlling glycerine tolerant weeds in glyc
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