The present invention relates to a dental floss holder, including: a holder body which has two arm portions and one grip portion connected with the two arm portions, wherein each front end of the two arm portions is provided with a through hole and two dental floss bolts penetratedly disposed in the two through holes respectively, wherein each dental floss bolt has a head portion protruding from the through hole, and a holding portion capable of holding the dental floss formed between an inner end surface of the head portion and an adjacent surface of the arm portion.本發明是關於一種牙線架,包含有:一架體,該架體具有二臂部,以及一聯結該二臂部的握持部,該二臂部的前端各設有一穿孔;二牙線栓,分別穿設在前述的二穿孔中,各具有一突出在該穿孔外的頭部,在該頭部的內端面與該臂部的鄰接面之間共同形成一可夾持牙線的夾持部位。(30)‧‧‧牙線架(31)‧‧‧架體(32)‧‧‧臂部(34)‧‧‧穿孔(341)‧‧‧容置部(40)‧‧‧牙線栓(41)‧‧‧頭部(42)‧‧‧尾端(43)‧‧‧外階面(44)‧‧‧內階面(45)‧‧‧桿部(50)‧‧‧襯套(51)‧‧‧肩部