The present invention provides a method of obtaining a classification boundary, to limit an axial depth in a puncturing operation. The following steps of method comprises: At first, obtaining a plurality of tomographic images from the axial depth of a tissue is performed. Then, obtaining a plurality of characteristic values from the tomographic images, the characteristic values are classified by a Support Vector Machine method. A classification boundary will be obtained through a distribution of the graph for defining a specific compartment of the tissue. In addition, an automatic recognition method and system using the above mentioned method are also disclosed in the present invention.本發明提供一種取得分類歸納邊界的方法,用以提供穿刺進行軸向深度的限制。其包含下列步驟:首先,取得一組織軸向深度上之複數個斷層影像。接著,於斷層影像中取得複數個特徵訊號值。最後,利用支持向量機分類法將該些特徵訊號值於一圖表表示,並由此圖表之分佈得出一分類歸納邊界以界定該組織之一特定區間。另外,應用上述方法之自動辨識方法及其系統亦揭露於本發明中。S302~S310b‧‧‧自動辨識方法