A stereo image pickup unit includes a first image pickup device 321 configured to receive a first optical image formed by a first objective optical system 311, a second image pickup device 32r configured to receive a second optical image formed by a second objective optical system 31r, a single centering glass 34 that is disposed on optical paths of the respective first and second optical images and to which light receiving surfaces 321a and 32ra of the respective first and second image pickup devices 321 and 32r are positioned and fixed through bonding, and a holding frame 35 that holds the first and second image pickup devices 321 and 32r through the centering glass 34.
