LANDRY DONALD,ZHAN CHANG-GUO,WOODS JAMES H.,SUNAHARA ROGER,NARASIMHAN DIWAHAR L.,MACDONALD JOANNE,YANG VICTOR,KO MEI-CHUAN HOLDEN,DENG SHI -XIAN,TESMER JOHN J.,LEE TIEN-YI,KWON YOUNG MIN,GAO DAQUAN
申请号：
NZ61292907
公开号：
NZ612929A
申请日:
2007.07.10
申请国别(地区):
NZ
年份:
2015
代理人:
摘要：
An isolated mutant cocaine esterase (CocE) polypeptide comprising an amino acid sequence at least 85% identical to SEQ ID NO: 1, wherein SEQ ID NO: 1 has at least one substitution, addition or deletion selected from the group consisting of L163V, V225I, I218L, A310D, A149S, S159A, S265A, S56G, W220A, S140A, F189L, A193D, T254R, N42V, V262L, L508G, Y152H, V160A, T172R, Y532F, T74S, W285T, L146P, D533S, A194R, G173Q, C477T, K531A, R41I, L119A, K46A, 10 F84Y, T172R-G173Q, L169K, F189A, N197K, R182K, F189K, V190K, Q191K, A194, I175-G-G-A186, and T176-G-G-D18 and the mutant CocE polypeptide has esterase activity with increased thermostability at 37°C as compared to wild-type CocE, wherein SEQ ID NO: 1 is disclosed within the specification.
