International AIDS Vaccine Initiative;University of Washington;The Scripps Research Institute
发明人:
Po-Ssu Huang,Joseph Graham Jardine,Sergey V. Menis,William Ray Schief, JR.,Neil P. King
申请号：
US14251132
公开号：
US20140322269A1
申请日:
2014.04.11
申请国别(地区):
US
年份:
2014
代理人:
摘要：
The invention relates to an engineered outer domain (eOD) of HIV gp120 and mutants thereof and methods of making and using the same. The mutant eODs may be advantageous for the elicitation of CD4-binding site (CD4bs)-directed broadly-neutralizing antibodies (bnAbs) and/or improve binding to mature VRC01 and/or improve binding to germline VRC01 and the germlines of other VH1-2 derived broadly-neutralizing antibodies. The mutant eODs may also include glycan-masking mutations on eOD. The present invention also includes fusions of eOD to various protein multimers to enhance immunogenicity as well as the design of cocktails of different eODs that represent the full diversity of HIV sequences within the VRC01 epitope and surroundings.
