A removable corral assembly for use in a livestock trailer which includes a plurality of interlocking livestock panels. The panels are in a substantial U-shape so when they are installed in the livestock trailer a left aisle and right aisle are formed with a middle or center aisle which is segregated. The worker stands in the center aisle and is protected from livestock being worked in the left and right aisles. A catch gate or squeeze chute may be associated with the corral assembly and positioned near the rear opening of the trailer in either the left or right aisle. Rollers, wheels or similar mechanisms are fastened to the panel assembly so that the corral can easily be rolled in to and out of a livestock trailer. Varying panel sizes may be used to adjust the overall dimensions of the corral assembly for installation in trailers having varying sizes.