A system and method for measuring thepotential eyestrain felt by audiences while watching a 3Dpresentation, e.g., a stereoscopic motion picture, areprovided. The eyestrain measurement system and method ofthe present disclosure is based on the measurement ofdisparity (or depth) and disparity transition of stereoscopicimages of the 3D presentation. The system and method ofthe present disclosure provide for acquiring a first imageand a second image from a first segment (202), estimatingdisparity of at least one point in the first image with atleast one corresponding point in the second image (204),estimating disparity transition of a sequence of first andsecond images (206), and determining potential eyestrainfelt while viewing the 3D presentation based on thedisparity and the disparity transition of the sequence of thefirst and second images (208).