The present invention provides a natural and non-harmful method for regulating and controlling a source and a library of rice. The library regulation and control are implemented by planting a sterile-series rice and a same-model maintenance-series rice thereof in a matching manner, or carrying out sowing in different periods and carrying out isolated pollination, so that rice plant samples whose sources and libraries have gradient difference horizontally are constructed. The present invention overcomes defects of physical injury or physiological interference exists in a leaf in cutting, ear cutting and other commonly used methods, and provides a new method and approach for deeply researching relationships of sources and libraries of crops such as rice, wheat and corns.
